Figure 5.

Right-angle light scattering confirms that Morin hydrate, but not Myricetin, inhibits IAPP amyloid formation. (A) Light scattering: solid line (—) IAPP alone, dashed line (----) IAPP plus Morin hydrate, and dashed-dotted line (–·–·–) IAPP plus Myricetin. (B) TEM image of the sample of IAPP without compound. Sample was removed at 42 h. (C) TEM image of a sample from the experiment conducted in the presence of Morin hydrate in fivefold excess. Sample was removed at 42 h. (D) TEM image of a sample from the experiment conducted in the presence of Myricetin in fivefold excess. Sample was removed at 42 h. All samples contained 32 μM IAPP, 0.25% DMSO (v/v) 20 mM Tris-HCl, pH 7.4. The signal was monitored at 500 nm. Experiments were conducted at 25°C. Scale bars in TEM images represent 100 nm. The light scattering data are the result of three separate measurements for each sample. The error bars represent the standard deviation of each data point.