Figure 6.

Morin hydrate disaggregates human IAPP amyloid fibers. (A) A thioflavin-T-monitored kinetic experiment in the absence of Morin hydrate. Morin hydrate was added in fivefold excess at the time point indicated by the black arrow. (B) TEM image of a sample removed just prior to the addition of Morin hydrate (black arrow). (C) TEM image 1 h after addition of Morin hydrate (red arrow). (D) TEM image 2 h after addition of Morin hydrate (green arrow). (E) TEM image 4 h after addition of Morin hydrate (dark blue arrow). (F) TEM image 6 h after addition of Morin hydrate (light blue arrow). Experiments were conducted at 25°C, pH 7.4, 20 mM Tris-HCl, 32 μM IAPP, 0.5% DMSO (v/v). The concentration of Morin hydrate was 160 μM. Scale bars represent 100 nm.