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The binding pocket for Nucleotide 5 and mutational result. (A) Close-up view of Nucleotide 5 binding pocket. Residues that interact with Nucleotide 5 are in surface representation and are indicated by labels. (B) Gel mobility shift result of PhCas6nc (1 μM) binding to Pf7, Pf7_guu, and Pf7_C5A RNAs. The upper shifted band is interpreted as the protein–RNA dimer and the lower shifted band is the protein–RNA monomer.
