Figure 4.

Static light scattering analysis of WT FGF-1 and a representative FGF-1 mutant as a function of temperature at the indicated pH values. (A) K12V/C117V/P134V, (B) WT FGF-1, and (C) WT FGF-1 with heparin. Data points are an average of two independent experiments. The complete static light scattering data set for the other nine FGF-1 mutants is provided in Supporting Information section. See Table I for a further description of each mutant including K12V/C117V/P134V (H in Table I). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]