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A: Thermal inactivation profiles and (B) thermal inactivation kinetics of wild-type lipase and selected single thermostable mutants upon incubation at 55°C. Inactivation profiles of other mutants are not shown for the sake of clarity. Symbols used: wild-type (•), A15S (–––), F17T (χ), G111S (◃), A132D (▾), M134R (▴), M134E (♦), M137P (▪), and N174T (⋄).
