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. 2012 Jun 15;3:105. doi: 10.3389/fphar.2012.00105

Figure 2.

Figure 2

Expression of ChETA-EYFP in the cerebral cortex of mice. (A) High resolution mosaic images of around 100 individual frames obtained from a 50-μm thick coronal section of PV::Cre mouse cerebral cortex after 14 days of rAAV infection. Level of expression in somatosensory cortex was controlled by fluorescence microscopy (EYFP filter). The red star indicates the injection site. TH, thalamus; fi, fimbria of hippocampus; vhc, ventral hippocampal commissure; CC, corpus callosum; GP, globus pallidus; IC, internal capsule; CPu, caudate putamen, S1FL, primary somatosensory forelimb cortex; S1BF, primary somatosensory barrel field cortex; S2, secondary somatosensory cortex; S1ULp, primary somatosensory upper limb cortex. (B) Diagram of a sagittal brain slice showing the injection site in the S1BF of the transgenic mouse brain. (C) The cell-type specificity of the viral expression was assayed using immunocytochemistry with an anti-parvalbumin antibody coupled to Alexa Fluor 568 (in red). EYFP expression was found to be largely restricted to parvalbumin expressing neurons (white arrow) in layer 2/3 of PV::Cre mouse somatosensory cortex.