FIGURE 8.

3-O-sulfation is required for efficient liver retention. Mice were injected via the tail vein with equal amounts of labeled heparin (black bar), 7-mer (11, white bar), decasaccharide without 3-O-sulfation (15a, light gray bar), or decasaccharide with 3-O-sulfation (15b, dark gray bar). After a short incubation time, mice were bled out, and livers were processed for scintillation counting. The data were measured by (cpm/mass of liver) of at least three liver samples from each mouse divided by the total cpm injected. Three mice were used for each construct (n = 3 ± S.E.).