TABLE 1.
Summary of the polysaccharide and oligosaccharide constructs
| Constructs | Anticipated disaccharide repeating unit | Size of the construct | Modification steps involved | 35S labeling site |
|---|---|---|---|---|
| 1 | (-GlcA-GlcNS-)n | Polysaccharide, >6,000 Da | NST | N-[35S]sulfation |
| 2 | (-GlcA-GlcNS6S-)n | Polysaccharide, >6,000 Da | NST and 6-OST | N-[35S]sulfation |
| 3 | (-IdoA-GlcNS6S-)n | Polysaccharide, >6,000 Da | NST, C5-epi, and 6-OST | N-[35S]sulfation |
| 4 | (-GlcA2S-GlcNS-)n | Polysaccharide, >6,000 Da | NST and 2-OST | N-[35S]sulfation |
| 5 | (-IdoA2S-GlcNS-)n | Polysaccharide, >6,000 Da | NST, C5-epi, and 2-OST | N-[35S]sulfation |
| 6 | (-IdoA2S-GlcNS6S-)n | Polysaccharide, >6,000 Da | NST, C5-epi, 2-OST, and 6-OST | N-[35S]sulfation |
| 7 | (-GlcA2S-GlcNS6S-)n | Polysaccharide, >6,000 Da | NST, 2-OST, and 6-OST | N-[35S]sulfation |
| 8 | (-GlcA-GlcNS ± 6S ± 3S-)n and (-IdoA2S-GlcNS6S-)m | Polysaccharide, >6,000 Da | NST, C5-epi, 2-OST, 6-OST, and 3-OST-1 | N-[35S]sulfation |
| 9 | (-GlcA2S-GlcNS6S-)n and (-GlcA-GlcNS ± 6S ± 3S-)m | Polysaccharide, >6,000 Da | NST, 2-OST, 6-OST, and 3-OST-1 | N-[35S]sulfation |
| 10 | Heparin, (-IdoA2S-GlcNS6S-)n and (-GlcA-GlcNS ± 6S ± 3S-)m | Polysaccharide, >6,000 Da | 3-OST-5 | 3-O-[35S]sulfation |
| 11 | GlcNAc6S-GlcA-GlcNS6S3S-IdoA2S-GlcNS6S-GlcA-AnMan | Heptasaccharide (7-mer) | Synthesis started from pure N-sulfo, N-acetylated heptasaccharide | 3-O-[35S]sulfation |
| 12a | GlcA-GlcNS6S- (IdoA ± 2S-GlcNS6S)2-GlcA-AnMan | Octasaccharide (8-mer no 3-O-sulfation) | Synthesis started from pure N-sulfo octasaccharide | N-[35S]sulfation |
| 12b | GlcA-GlcNS6S ± 3S- (IdoA ± 2S-GlcNS6S)2-GlcA-AnMan | Octasaccharide (8-mer with 3-O-sulfation) | Synthesis started from pure N-sulfo octasaccharide | N-[35S]sulfation |
| 13a | GlcNS6S-GlcA-GlcNS6S- (IdoA ± 2S-GlcNS6S)2-GlcA-AnMan | Nonasaccharide (9-mer no 3-O-sulfation) | Synthesis started from pure N-sulfo nonasaccharide | N-[35S]sulfation |
| 13b | GlcNS6S-GlcA-GlcNS6S ± 3S- (IdoA ± 2S-GlcNS6S)2-GlcA-AnMan | Nonasaccharide (9-mer with 3-O-sulfation) | Synthesis started from pure N-sulfo nonasaccharide | N-[35S]sulfation |
| 14 | GlcA-GlcNS6S ± 3S- (GlcA ± 2S-GlcNS6S)3-GlcA-AnMan | Decasaccharide (10-mer no IdoA) | Synthesis started from pure N-sulfo decasaccharide | 6-O-[35S]sulfation |
| 15a | GlcA-GlcNS6S- (IdoA ± 2S-GlcNS6S)3-GlcA-AnMan | Decasaccharide (10-mer no 3-O-sulfation) | Synthesis started from pure N-sulfo decasaccharide | 6-O-[35S]sulfation |
| 15b | GlcA-GlcNS6S ± 3S- (IdoA ± 2S-GlcNS6S)3-GlcA-AnMan | Decasaccharide (10-mer with 3-O-sulfation) | Synthesis started from pure N-sulfo decasaccharide | 6-O-[35S]sulfation |
| 16 | GlcA-GlcNS6S ± 3S- (GlcA ± 2S-GlcNS6S)4-GlcA-AnMan | Dodecasaccharide (12-mer no IdoA) | Synthesis started from pure N-sulfo dodecasaccharide | 6-O-[35S]sulfation |
| 17a | GlcA-GlcNS6S- (IdoA ± 2S-GlcNS6S)4-GlcA-AnMan | Dodecasaccharide (12-mer no 3-O-sulfation) | Synthesis started from pure N-sulfo decasaccharide | 6-O-[35S]sulfation |
| 17b | GlcA-GlcNS6S ± 3S- (IdoA ± 2S-GlcNS6S)4-GlcA-AnMan | Dodecasaccharide (12-mer with 3-O-sulfation) | Synthesis started from pure N-sulfo decasaccharide | 6-O-[35S]sulfation |
| 18 | GlcNS6S- (GlcA ± 2S-GlcNS6S ± 3S)3- (GlcA-GlcNAc6S)2-GlcA-GlcNS6S-GlcA-AnMan | Pentadecasaccharide (15-mer no IdoA) | Synthesis started from pure N-sulfo, N-acetylated pentadecasaccharide | 6-O-[35S]sulfation |
| 19 | GlcNS6S- (IdoA ± 2S-GlcNS6S ± 3S)3- (GlcA-GlcNAc6S)2-GlcA-GlcNS6S-GlcA-AnMan | Pentadecasaccharide (15-mer) | Synthesis started from pure N-sulfo, N-acetylated pentadecasaccharide | 6-O-[35S]sulfation |
| 20 | GlcNAc6S- (GlcA ± 2S-GlcNS6S ± 3S)4- (GlcA-GlcNAc6S)2-GlcA-GlcNS6S-GlcA-AnMan | Heptadecasaccharide (17-mer no IdoA) | Synthesis started from pure N-sulfo, N-acetylated heptadecasaccharide | 6-O-[35S]sulfation |
| 21 | GlcNAc6S- (IdoA2S-GlcNS6S ± 3S)4- (GlcA-GlcNAc6S)2-GlcA-GlcNS6S-GlcA-AnMan | Heptadecasaccharide (17-mer) | Synthesis started from pure N-sulfo, N-acetylated heptadecasaccharide | 6-O-[35S]sulfation |
| 22 | GlcNS6S- (IdoA2S-GlcNS6S ± 3S)5- (GlcA-GlcNAc6S)2-GlcA-GlcNS6S-GlcA-AnMan | Nonadecasaccharide (19-mer no IdoA) | Synthesis started from pure N-sulfo, N-acetylated nonadecasaccharide | 6-O-[35S]sulfation |
| 23 | GlcNS6S- (IdoA2S-GlcNS6S ± 3S)5- (GlcA-GlcNAc6S)2-GlcA-GlcNS6S-GlcA-AnMan | Nonadecasaccharide (19-mer) | Synthesis started from pure N-sulfo, N-acetylated nonadecasaccharide | 6-O-[35S]sulfation |