TABLE 2.

Enzymatic activity and assembly of Tk-R15Pi and its mutants

The molecular assembly of these proteins was examined by size exclusion chromatography (Fig. 3 and supplemental Fig. 10).

Tk-R15Pi	Specific activitya
	μmol min−1mg−1
WT	29.3 ± 2.1
C133S	<0.1
C133A	<0.1
D202N	<0.1
R227E	0.5 ± 0.2

^a R15P enzymatically synthesized by AMP phosphorylase was used as the substrate.