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. 2012 Apr 17;287(25):20784–20796. doi: 10.1074/jbc.M112.349423

TABLE 3.

Enzymatic activity of Tk-R15Pi

Substrate Specific activitya Keq = [RuBP]/[R15P]b
μmol min1mg1
α-R15P 28.6 ± 1.1 16.3 ± 0.2
β-R15P <0.1

a Chemically synthesized R15Ps were used as initial substrates.

b Keq was calculated by integration of the peak area from the HPLC experiments (supplemental Fig. 7C).