FIGURE 3.

RyR and IP₃R participation in [Ca²⁺]_rest in WT and mdx myotubes. A, effect of Ry (30 μm), Ry + B5 (Ry, 30 μm, and B5, 10 μm), U-73343 (5 μm), U-73122 (5 μm), and XeC (5 μm) on [Ca²⁺]_rest (treatments for 3 h). B, representative traces of Fluo-4 fluorescence signals after the addition of 5 μm ionomycin, in the absence of extracellular Ca²⁺ (Ca²⁺-free solution) in WT, mdx, and Ry- and XeC-treated mdx myotubes. C, average area under the curve of the ionomycin-induced Ca²⁺ transients. Data are expressed as means ± S.E. ***, p < 0.001; **, p < 0.01; *, p < 0.05 versus WT basal value, †, p < 0.05; †††, p < 0.001 versus mdx basal value. §§§, p < 0.001 is indicated in the figure.