FIGURE 6.

Effects of Nrf2 Y286A mutation on Cav-1-Nrf2 reaction and subsequent cellular antioxidant capacity. A, Beas-2B cells were transfected with control vector, pcDNA3-EGFP-Nrf2, or Y286A mutated Nrf2 (Mu-Nrf2) for 48 h and were harvested for immunoprecipitation (IP) or Western blot (IB) analysis as indicated. B, Beas-2B cells were co-transfected with the antioxidant response element reporter plasmid and the indicated cDNA for 48 h and were harvested for luciferase assay. RLU, relative luciferase unit. C and D, Beas-2B cells were transfected with control vector, pcDNA3-EGFP-Nrf2 (WT-Nrf2), or Y286A mutated Nrf2 (Mu-Nrf2) for 48 h and followed by 15 μm of 4-HNE treatment for 6 h for HO-1 mRNA expression (C) or various concentrations of H₂O₂ for 24 h for cell viability assay (D). *, significantly different from the corresponding value of vector control (p < 0.05); #, significantly different from the corresponding value of WT-Nrf2 (p < 0.05).