Summary of the sites of O-GalNAc addition by ppGalNAc-T1, -T3, and -T5 on peptide and O-mannosylated glycopeptides from regions of α-DG detected by mass spectrometry. Sites of preinstalled O-Man are indicated by filled green circles and sites of O-GalNAc addition by the ppGalNAc-T enzymes are denoted by filled yellow squares. A, results for the PPTTTTKKP-derived peptides and glycopeptides, and B, results for the RIRTTTSGVPR-derived peptides and glycopeptide.