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8-Br-cGMP (10 μm) induces a 2-fold greater relaxation of the submaximal Ca2+-activated force in α-toxin permeabilized ileum from WT compared with telokin-null mice (upper panel) in the absence of a commensurate difference in MYPT1 Thr-696 and Thr 853 phosphorylation as shown in the typical Western blots and bar graph (lower two panels). *, p < 0.05; n.s., not significant.
