FIGURE 6.
PDZK1-mediated ternary complex is required for PLC-β3-dependent Ca2+ signaling. A, HEK293 cells that stably express SSTR5 or its PDZ-binding motif deletion mutant (SSTR5ΔC) were loaded with Fura-2/AM and subsequently stimulated with 5 μm SST. B, HEK293 cells that stably express SSTR5 were transiently transfected with scrambled (siControl) or PDZK1 siRNA (siPDZK1). The cells were loaded with Fura-2/AM and then stimulated with 5 μm SST. C, HEK293 cells that stably express SSTR5 were transiently transfected with the control, PLC-β1, or PLC-β3 shRNA. The cells were loaded with Fura-2/AM and then stimulated with 5 μm SST. The changes in intracellular Ca2+ concentration ([Ca2+]i) were monitored. The results shown are representative of three independent experiments, and the data represent the means ± S.D. (error bars) (*, p < 0.05; **, p < 0.01).