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. 2012 May 3;287(25):21110–21120. doi: 10.1074/jbc.M111.319129

FIGURE 5.

FIGURE 5.

Genetic knockdown of autophagy specific genes rescued cells from PA-induced cell death. A, knockdown efficiency of individual siRNA in the percentage of mRNA expression compared with scramble siRNA (n = 3 for all columns presented). *, p < 0.05 versus scrambled siRNA. B, 48 h after cells transfection with either scrambled siRNA (left pair of columns, n = 9 for both conditions) or siRNA against VPS34 (right pair of columns, n = 9 for both conditions) cells were incubated for 24 h with BSA alone (white columns) or 0.5 mm PA complexed to BSA (black columns) and cell viability was measured using the MTT assay. Viability is expressed in percentage, whereas the average value of cells treated with scrambled siRNA and BSA alone was defined as 100%. *, p < 0.05 versus BSA, and #, p < 0.05 versus PA with scrambled siRNA. C, impact of siRNA-mediated knockdown of ATG7 on cell viability under analogous conditions described in panel b (n = 9 for each column). *, p < 0.05 versus BSA, and #, p < 0.05 versus PA with scrambled siRNA.