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. 2012 Apr 18;287(25):21121–21129. doi: 10.1074/jbc.M112.341016

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Repeated polymerization-depolymerization cycles. A, Alp12 (8 μm) could be cycled repeatedly through polymerization and depolymerization. At each green arrow, 100 μm NTP was added. At the purple arrows, 1 mm NTP was added. Red, ATP; blue, GTP. B, ATP and GTP were not completely exchangeable. The amount of nucleotide added 100 μm ATP (green arrows), 100 μm GTP (purple arrow), 1 mm GTP (orange arrow), and 1 mm ATP (blue arrow). Note that 100 μm GTP was not sufficient to repolymerize Alp12. The Alp12 concentration was 20 μm.