FIGURE 5.

FXYD domains involved in surface expression. Oocytes were either not injected (NI) or injected with cRNA mixtures coding for HA-tagged PLM (2 ng) or FXYD7 (2 ng) ± α1(10 ng) and β1 (7 ng) Na⁺/K⁺ ATPase (NaK). Additional groups of oocytes were injected with 2 ng of two PLM/FXYD7 chimeras. One had the extracellular domain of HA-FXYD7 but the transmembrane and intracellular segments of PLM (7PP). The other had the extracellular and transmembrane domains of HA-PLM and the intracellular segment of FXYD7 (PP7). Surface expression of the HA tag was measured 3 days later, and the data are means ± S.E. of 24 oocytes. Data are expressed as a fraction of the mean chemiluminescence signal in oocytes expressing HA-FXYD7.