Chimera construction of the GlyR α1 and β subunits. Sequences of GlyR α1 and β subunits are in gray and black, respectively. The abbreviations are as follows: L2, loop 2; Ld, loop D; La, loop A; Le, loop E; Cy, Cys-loop; Lb, loop B; Lf, loop F; Lc, loop C; Pm, pre-M1; TMD, transmembrane domain.