Obst-A is essential for larval growth and survival.
A, brightfield microscopy images of obst-A-null mutants and heterozygous control larvae. obst-A mutant larvae fail to increase their body size, when compared with control animals. Scale bars, 0.5 mm. B, survival tests revealed larval lethality of obst-A-null mutants, but not for wt and control animals (0–86 h after egg laying (AEL); obst-Ad02 n = 137; obst-Ad03 n = 128, wt n = 30; heterozygous obst-Ad02/FM7 n = 60; heterozygous obst-Ad03/FM7 n = 91). C, immunoblot treated with the anti-Obst-A antibody detects Obst-A (∼26,5kDa predicted) in wt embryo and larval extracts.