FIGURE 4.

Potassium efflux antiporters are not part of the TGD complex. A, 35-day-old Arabidopsis are shown with genotypes labeled above. B, genomic DNA extracted from genotypes listed above (N, no-DNA control) was genotyped using PCR with primers recognizing alleles indicated at the right (KEA1 is the wild-type KEA1 allele, and kea1 is the T-DNA insertion into KEA1; KEA2 and kea2 for the KEA2 allele are designated accordingly). C, total fatty acids of lipids converted to fatty acid methylesters and quantified by gas chromatography for genotypes listed above. The S.D. value of three biological replicates is shown (error bars). D, dodecylmaltoside-solubilized leaf proteins from Arabidopsis genotypes listed at the left were separated in the first dimension by 4–14% HDN-PAGE and in the second dimension by 12% SDS-PAGE and then immunoblotted and detected with TGD2 antiserum.