TABLE 1.

Proteins identified by mass spectrometry

Gel bands from which proteins were identified are indicated at the left. The major P. sativum (pea) contig identified is given, as is the annotated Arabidopsis homolog's accession number. Total spectral counts (SpC), unique peptides, molecular masses (kDa), and percentage coverage are given for each of three biological replicates (numbered 1–3), based on 99% protein identification and 95% peptide identifications. Molecular masses and lengths used to calculate percentage coverage are based on predicted mature Arabidopsis homologs to reduce variations due to pea contig lengths and overlap. Pea contigs without Arabidopsis homologs were assigned the molecular mass of the band and the percentage coverage of the contig. APE2, acclimation of photosynthesis to environment 2; CLP, caseinolytic protease; LSU R, large subunit of Rubisco; PORA, protochlorophyllide oxidoreductase; TIC, translocon at the inner envelope membrane of chloroplasts; TIM, translocase at the inner mitochondrial membrane; TOC, translocon at the outer envelope membrane of chloroplasts; WPP, Trp-Pro-Pro motif.

Band no.	A. thaliana accession no.	Pea contig	Name	SpC			Mass	Unique peptides			Percentage coverage
				1	2	3		1	2	3	1	2	3
							kDa				%	%	%
1	At4g00630	Ps066597f	K+ efflux antiporter (KEA2)	45	33	21	126.2	19	12	8	18.7	10.7	8.4
	At5g23890	Ps000222b	Putative transporter	21			103.9	7			10.1
	At4g34830	Ps027447f	Maturation of LSU R 1	11			119.8	5			6.6
	At4g02510	Ps007362a	TOC159	1	10	1	160.8	1	6	1	0.8	8.2	1.6
	At1g06950	Ps023494a	TIC110		37		112.1		18			25.7
	At3g46740	Ps022977c	TOC75		1		75		1			2.9
	At2g28000	Ps010082c	Chaperone, CPN60a	2			62.07	2			5.6
	At3g13470	Ps009941b	Chaperone, CPN60b-2	2			63.34	2			4.7
	N/A	Ps070470a	No annotation	6		2	140	4		2	78.8		59.6
	N/A	Ps039763e	No annotation	2		3	140	2		2	21.0		21.0
	N/A	Ps054876c	No annotation	3			140	2			51.4
	N/A	Ps033657a	No annotation	3			140	2			15.7
2	At3g20320	Ps000114b	TGD2	133	92	100	36	43	34	32	77.4	68.5	73.0
	At1g65410	Ps016716e	TGD3	25	16	27	36	14	7	12	55.1	27.6	45.2
	At5g05000	Ps013223b	TOC34	48		9	34.71	18		5	42.5		14.7
	At3g47520	Ps007032f	Malate dehydrogenase	3	10	16	42.41	3	5	8	9.9	10.5	30.9
	At5g42130	Ps002665b	Putative transporter	3			44.36	2			9.2
	At5g54190	Ps005013a	PORA	3		4	43.86	2		3	8.5		14.4
	At5g01500	Ps017159a	Thylakoid ATP carrier	1			45.09	1			5.1
	At4g04640	Ps017279a	ATP synthase g chain 1		3		40.91		3			11.7
	At3g63490	Ps013200a	Ribosomal L1 family protein		2	7	37.63		2	3		9.4
3	At1g19800	Ps023468d	TGD1	14	8	10	28	6	4	6	24.0	17.7	24.0
	At5g46110	Ps006793e	APE2	11			44.63	5			9.6
	At2g37220	Ps002377c	Ribonucleoprotein,putative	4		5	30.72	3		2	14.8		14.4
	At5g24650	Ps010239c	TIM17/22/23 family protein	4	9	11	27.77	2	3	4	10.0	16.6	20.1
	At3g25920	Ps010043a	Ribosomal protein L15	5		4	29.71	4		2	21.1		10.8
	At2g43030	Ps002441d	Ribosomal L3 family protein	3		14	29.36	2		7	12.6		43.9
	At5g43070	Ps015737a	WPP domain protein 1	2			16.63	1			16.5
	Atcg00490	Ps021077b	Small subunit of RUBISCO	2			26.91	2			4.2
	Atcg00160	Ps069182c	Ribosomal protein S2	2			52.96	2			12.3
	At3g27160	Ps001242c	Ribosomal protein S21			5	20.91			2			15.4
	At1g49970	Ps029444f	CLP protease subunit 1			4	42.63			2			6.6
	At3g20320	Ps000114b	TGD2		3	2	35		1	1		3.6	3.6
	At3g13470	Ps009941b	Chaperone, CPN60b-2	1			63.34	1			2.4