Table I.

K_m and k_cat values for bacterial ADPRTs

Values for kinetic parameters of SpyA were determined as described in the legend to Fig. 1. All values were determined in the presence of excess substrate and variable [ϵ-NAD⁺]. Data represent the mean ±S.D. of at least three independent experiments. ND, not determined. DT, diphtheria toxin; PT, pertussis toxin; SBTI, soybean trypsin inhibitor; eEF-2, eukaryotic elongation factor 2.

Bacteria	Toxin	Substrate(s)	Km	kcat	Reference/Source
			μm	min−1
S. pyogenes	SpyA	Vimentin	76.2 ± 9.3	58.5 ± 3.4	This paper
		Actin	79.6 ± 3.5	10.1 ± 0.6
Photorhabdus luminescens	Photox	Actin	27.4 ± 4.4	1,680 ± 75	Ref. 41
Clostridium botulinum	C2Ia	Actin	21.3 ± 1.2	ND	Ref. 74
Clostridium perfringens	Iota aa	Actin	3.8–6.0	2,024 ± <15%	Refs. 61 and 74
Salmonella enterica	C/SpvBa	Actin	4.4 ± 0.6	ND	Ref. 74
C. botulinum	C3bot	RhoA	8	15b	Ref. 75
Clostridium limosum	C3lim	RhoA	52 ± <15%	0.28 ± <15%	Ref. 76
Bacillus cereus	C3cer	RhoA	20.8 ± 1.6	22.25 ± 0.9	Ref. 77
		RhoB	56.4 ± 3.6	4.19 ± 0.65
		RhoC	17.1 ± 3.3	0.99 ± 0.08
P. aeruginosa	ExoS	SBTI	30 ± 10	25 ± 20	Refs. 78 and 79
		Ras	9	ND
P. aeruginosa	ExoA	eEF-2	275 ± 52	675 ± 85	Ref. 42
Corynebacterium diphtheriae	DT	eEF-2	2–9	58.8–182	Refs. 80–82
Bordetella pertussis	PT	αi3C20c	27.0	3.0	Refs. 83 and 84
		Giαd	23.0	40.0

^a C2I and ι a are the enzymatic components of C2 and ι toxin, respectively. C/SpvB is the C terminal enzyme domain of SpvB.

^b Calculated from V_max from Ref. 75.

^c C-terminal 20 amino acids of the α-subunit of the pertussis toxin target G-protein G_i3.

^d α-Subunit of the pertussis toxin target G_i1 protein.