FIGURE 1.

Selective overexpression of PFKFB3/iPFK2 in adipose tissue protects mice from diet-induced insulin resistance. A, genomic DNA was prepared from Adi-PFKFB3TG (Tg) mice and wild-type littermates and used for PCR analyses of aP2-PFKFB3 transgene. B, iPFK2 (encoded by PFKFB3) abundance in white adipose tissue (WAT), liver, skeletal muscle, and bone marrow (BM) was measured using Western blots. C, insulin tolerance tests. D, glucose tolerance tests. C and D, male Tg mice and WT mice, at 5–6 weeks of age, were fed an HFD for 12 weeks. After the feeding regimen, HFD-fed mice were fasted for 4 h and received an intraperitoneal injection of insulin (1 unit/kg) (C) or glucose (2 g/kg) (D). Data are means ± S.E., n = 6–10. ^†, p < 0.05; ^††, p < 0.01 Tg versus WT at the same time point.