FIGURE 6.

Hydrolysis of oxidized forms of deoxyribonucleoside diphosphates by MTH3. The reaction mixture (10 μl) contained 20 mm Tris-HCl (pH 8.0), 80 μg/ml BSA, 8 mm MgCl₂, 40 mm NaCl, 5 mm DTT, 2% glycerol, 30 ng of MTH3 protein, and 10 μm nucleoside diphosphate (8-oxo-dGDP, 2-OH-dGDP, or 8-OH-dADP). The mixture was incubated at 30 °C, and the reaction was terminated by the addition of SDS (final concentration, 1%). A, HPLC elution profiles of the nucleotides before and after treatment for 4 or 12 min. B, time-dependent increases of the degradation products.