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Kinetic parameters of MTH3 on 8-oxo-Gua-containing nucleotides
The reactions were performed at different concentrations of substrates, 2.2–20 μm 8-oxo-dGDP and 8-oxo-GDP, using 10 ng of MTH3 protein in Tris-HCl buffer (pH 8.0) as described under “Experimental Procedures.” The assays were done in triplicate, and the means ± S.D. are shown.
| Substrate | Km | Vmax | Vmax/Km |
|---|---|---|---|
| μm | pmol/min/μg | ||
| 8-Oxo-dGDP | 10.7 ± 3.1 | 212 ± 30 | 19.8 ± 9.7 |
| 8-Oxo-GDP | 11.7 ± 2.9 | 246 ± 49 | 22.4 ± 16.9 |
