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. 2012 May 1;287(25):21550–21560. doi: 10.1074/jbc.M112.348607

FIGURE 4.

FIGURE 4.

Effect of Gαs, Gαq/11, and Gα12/13 subfamily knockdown on GnRH-induced gonadotropin subunit transcription. LβT2 cells were transfected with control or Gα siRNA by nucleofection. Three days after siRNA transfection, cells were stimulated with either vehicle (no GnRH) or 1 nm GnRH for 2 h, followed by a 4-h incubation without GnRH, to induce gonadotropin subunit expression. The effect of Gαs, Gαq/11, and Gα12/13 knockdown on GnRH-induced gonadotropin subunit expression was determined by real-time PCR analysis. For statistical analysis, two-way ANOVA with Bonferroni post test was used. ***, p < 0.001; **, p < 0.01; *, p < 0.05. Error bars, S.E.