FIGURE 9.

Inhibin expression is regulated in a GnRH pulse frequency-specific manner. LβT2 gonadotrope cells were stimulated with vehicle or high or low frequency GnRH pulses using a custom designed cell perfusion system. For high pulse frequency GnRH stimulation, gonadotrope cells were exposed to 5 min of 5 nm GnRH pulses every 30 min for a 10-h period; and for low pulse frequency GnRH stimulation, cells were exposed to 5 min of 5 nm GnRH pulses every 2 h for a 10-h period. The levels of FSHβ and LHβ (A) and inhibinα (B) mRNA expression were monitored by real-time PCR analysis. For statistical analysis, one-way ANOVA with Bonferroni post test was used. ***, p < 0.001; **, p < 0.01; *, p < 0.05. Error bars, S.E.