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Acute alcohol withdrawal (24 h after the vapor was turned off) was accompanied by GR downregulation in stress/reward-related brain areas. Dependent rats showed lower GR mRNA levels in the (a) PFC, (b) NAc, and (c) BNST but not (d) amygdala or (e) hippocampus compared with nondependent rats. Insets illustrate the approximate location of brain punches. Data represent mean and SE. *p < 0.05, significant difference between dependent and nondependent. n = 7–17 per group.
