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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Gene Ther. 2012 Jun;19(6):588–595. doi: 10.1038/gt.2012.33

Figure 2.

Figure 2

(a) Top: representative tracings of HCN-encoded pacemaker current from Ad-CMV-GFP-, Ad-CMV-WT-HCN1-, Ad-CMV-HCN1-ΔΔΔ- and Ad-CMV-HCN1-QQ253QQ-transduced ventricular CMs. Bottom: automaticity could be recorded only from the Ad-CMV-HCN1-ΔΔΔ-transduced group. Steady-state activation curves indicate that HCN1-ΔΔΔ channels open most easily. Correlation of HCN current to induced pacing rate, MDP and phase 4 slope of HCN1-ΔΔΔ-mediated biopacemaking. (b) Effects of the If blocker ZD7288 on WT or engineered HCN1-transduced LV CMs. Spontaneously firing APs recorded from an HCN1-ΔΔΔ-transduced LV CM are abolished after addition of ZD7288. Upon silencing, an electrical stimulus elicits a normal ventricular AP. Adapted from Xue et al.49