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FRET and two-state protein folding. (a) Kinetic scheme that describes the folding and unfolding of a protein with attached donor and acceptor dyes. (b) The conformational trajectory which is of interest but invisible. (c) The observed photon trajectory when the arrival time of each detected photon is recorded. (d) A photon trajectory when only the numbers of acceptor and donor photons in a time bin of duration T is recorded.
