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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Drug Deliv Transl Res. 2011 Jun 1;1(3):209–222. doi: 10.1007/s13346-011-0022-6

Figure 8.

Figure 8

Weighed pieces of dapivirine films were dissolved in either DMEM (for cell-based assays) or 1X RT-buffer (for in vitro RT assays) at 1:10 w/v. Multiple dilutions of the starting solutions were then prepared and used. For the RT assay, inhibition of the RNA-dependent DNA polymerase activity of HIV-1 RT was determined using poly(rA)-oligo(dT) and [3H-TTP]. Cell-based single HIV-1 replication cycle assays for direct antiviral activity of the film formulations used P4R5 indicators cells as described above. Inhibition of cell-based HIV-1 replication by dapivirine film (0.5 mg/film) is over 200-fold more potent than inhibition of RT activity.