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Gel filtration chromatogram of the wild-type and E134A mutant HaNDKs. Approximately 100 μg of wild-type HaNDK (1 mg mL−1) and of the E134A mutant HaNDK (1 mg mL−1) were injected onto SK-GEL G3000 SWXL column equilibrated with 50 mM Tris HCl buffer at pH 8.0, containing 2 mM MgCl2 and (A) 0.2M NaCl or (B) 2.0M NaCl. Approximately 100 μg of the bovine serum albumin (1 mg mL−1) was also analyzed as a standard sample.
