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. 2012 Jan 23;21(4):498–510. doi: 10.1002/pro.2032

Figure 3.

Figure 3

Monomeric structure and subunit assembly of wild-type and E134A mutant HaNDKs. (A), the wild-type HaNDK monomer. (B), the E134A mutant HaNDK monomer. In (A) and (B), 134th residues are shown in sticks. Kpn loop is shown in dotted circle. (C), the wild-type HaNDK dimer. (D) and (E), the E134A mutant HaNDK tetramer. The tetramers (D) and (E) are called Type I and II tetramers in this article. (F), Crystal packing in the asymmetric unit of the E134A mutant HaNDK (Form 3). Eight subunits (a–h) were observed in the asymmetric unit of Form 3 crystal. There are two kinds of tetrameric assembly (Type I and II tetramers) in the asymmetric unit of the E134A mutant HaNDK. Tetrameric comformation of Type I tetramer is similar to that of Myxococcus NDK (MxNDK). On the other hand, tetrameric conformation of Type II tetramer is similar to that of E. coli NDK (EcNDK). Red circles in (F) show the dimmer–dimer interfaces in Type I tetramer including mutation site (E134A). Black circles in (F) show the Kpn loops.