Table I.
Molecular Mass of the Wild-Type and E134A Mutant HaNDKs Determined Using Light Scattering Analysis
| Theoretical mass (Da) | ||||||
|---|---|---|---|---|---|---|
| Sample | NaCl in eluting solvent (M) | Observed mass (Da) | Monomer | Dimer | Tetramer | Assignment |
| Wild-type HaNDK | 0.2 | 33,700 ± 2000 | 15,137 | 30,274 | 60,548 | Dimer |
| 2.0 | 32,100 ± 2200 | |||||
| E134A mutant HaNDK | 0.2 | 62,100 ± 4300 | 15,076 | 30,158 | 60,316 | Tetramer |
| 2.0 | 65,300 ± 5200 | |||||
| BSA (standard sample) | 0.2 | 70,800 ± 7400 | 66,776 | — | — | Momomer |
| 2.0 | 72,600 ± 2900 | |||||
The theoretical mass was calculated from amino acids sequence. Assignment of multimer was determined by comparison between observed and theoretical mass.