Table II.

Hydrogen Bonds in the Wild-Type and E134A Mutant HaNDKs

Domain	Residue	Atom	Distance, Å	Atom	Residue	Domain
Monomer–monomer interface of the wild-type HaNDK dimer (Form 1)
α1	V15	O	3.3	Nη1	R141	C-terminal
α2	N18	Oδ1	2.2
		O	3.2	Oε1	E28	α2
	I20	N	3.2	Oε1
			3.3	Oε2
	G21	N	2.9	Oε1
		O	3.4	O	G21
	E22	N	3.2	Oγ	S25
	S25	Oγ	3.3	N	E22
	E28	Oε1	2.7	N	G21
			3.3	O	N18
			3.2	N	I20
		Oε2	3.2	O	N18
			3.1	N	I20
β2	A37	O	2.8	N	M39	β2
	M39	N	3.0	O	A37
C-terminal	R141	Nη1	3.1	O	V15	α1
			2.7	Oδ1	N18	α2
Dimer–dimer interface of the E134A mutant HaNDK (Type I tetramer in Form3)

Chain	Residue	Atom	Distance, Å	Atom	Residue	Chain
A	E135	N	2.9	O	A130	b
	S136	N	3.1	O	Y131
		Oγ	2.8
	A103	O	2.9	N	E135
	Y131	O	3.0	N	S136
			3.0	Oγ
E	E135	N	2.9	O	A130	f
			3.3	O	Y131
	S136	N	2.9
		Oγ	3.2
	A130	O	2.8	N	E135
	Y131	O	3.1	N	S136
			2.7	Oγ
Dimer–dimer interface of the E134A mutant HaNDK (Type II tetramer in Form3)
B	K29	Nζ	3.2	Oε1	E22	c
	D88	Oδ1	3.3	Oε2	E99
f	G101	O	2.9	Nη2	R26	g
			3.3	Nη1

Names of chains are as in Figure 3(F).