Table III.
Samples | Source | Mutation | Amino acid sequence from 131th | Assembly (cross link)a | Assembly (SEC-MALTTS) | X-ray structure |
---|---|---|---|---|---|---|
HaNDK | Halophiles | None (wild-type) | YFFEESEICSR | 2 | 2 | 2.3 Åb |
Halophiles | E135A | YFFEASEICSR | 2 | |||
Halophiles | E134A | YFFAESEICSR | 4 | 4 | 2.3 Åb | |
Halophiles | E134A E135A | YFFAASEICSR | 4 | |||
PaNDK | non-Halophiles | None (wild-type) | YFFAATEVCERIR | 4 | 4 | |
non-Halophiles | A135E | YFFAETEVCERIR | 4 | |||
non-Halophiles | A134E | YFFEATEVCERIR | 2 | |||
non-Halophiles | A134E; A135E | YFFEETEVCERIR | 2 | |||
MxNDK | non-Halophiles | None (wild-type) | YFFRETEIHSYPYQK | 2.0 Åc |