Figure 3.

Protein solubility in various over-expression backgrounds and temperatures. Determination of protein solubility of sRI (A) and sT (B) in various over-expression backgrounds and temperatures. T: total sample, P: insoluble fraction, and S: soluble fraction. Molecular mass standards used are indicated on left. A: Western blot of sRI samples over-expressed at 37°C in BL21(DE3), Origami(DE3), and both 37 and 16°C in the SHuffle(DE3) backgrounds. B: Western blots of sT samples over-expressed at 16 and 37°C in the SHuffle(DE3) background. C: Coomassie-stained gel of samples in which sT was eluted with or without the presence of sRI. Migration of sRI, * and sT, ** are indicated.