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. 2012 Feb 9;21(4):571–582. doi: 10.1002/pro.2042

Figure 4.

Figure 4

Secondary structure prediction and CD spectroscopy. A: Secondary structure prediction of sRI by JPred. α-helices are indicated by ovals and unstructured elements indicated by lines. The location of the two cysteine residues are indicated by arrows and the C-terminal oligohistidine tag is labeled. sRI is predicted to contain 65% alpha-helical content with no other structured elements predicted. B: Secondary structure prediction of sT by JPred. α-helices are indicated by ovals, β-sheets are indicated by large arrows, and unstructured elements are indicated by lines. The location of the two-cysteine residues is indicated by arrows and the C-terminal oligohistidine tag is labeled. sT is predicted to contain 30.5% alpha-helical character, and 19% beta-sheet with no other structured elements predicted. C: CD spectra of sRI (•) and sT-sRI complex (▪) at a final concentration of 1 μM each. The difference spectrum (of sRI and the sT-sRI complex) is indicated (□).