Figure 2.

In situ magic angle spinning solid state NMR ¹⁹F chemical shifts (A, C, E) and longitudinal relaxation T₁ values (G, I, K) were obtained for the DAGK protein in its native E. coli membrane without protein purification. Solution NMR ¹⁹F chemical shifts (B, D, F) and longitudinal relaxation T₁ values (H, J, L) were obtained for samples of purified DAGK in DPC micelles. Three ¹⁹F-tfmF site-specifically labeled DAGK proteins were presented: F31-tfmF (A, B, G, H); L48-tfmF (C, D, I, J) and L116-tfmF (E, F, K. L).