Table 2.
Immunoreagents, working dilutions and sources of antibodies used in the present study.
| Primary antibody | Working dilution | Antibody suppliers (Reference) |
|---|---|---|
| Mouse anti-glutamine synthetase monoclonal antibody | 1 : 200 | Chemicon (Ref. MAB302) |
| Mouse anti-Islet-1 monoclonal antibody (clone 39.4D5) | 1 : 5 | Developmental Studies Hybridoma Bank (DSHB) |
| Mouse anti-SV2 monoclonal antibody | 1 : 10 | DSHB |
| Mouse anti-TUJ1 monoclonal antibody | 1 : 100 | Abcam (Ref. ab14545) |
| Rabbit anti-bovine rod opsin polyclonal antibody, CERN-922 | 1 : 1000 | Gift from Dr Willem J. DeGrip |
| Rabbit anti-calbindin polyclonal antibody | 1 : 1000 | Swant (Ref. CB-38a) |
| Rabbit anti-calretinin polyclonal antibody | 1 : 2000 | Swant (Ref. 7699-4) |
| Rabbit anti-Goα polyclonal antibody | 1 : 500 | Millipore (Ref. 07-634) |
| Rabbit anti-phospho-Histone H3 polyclonal antibody | 1 : 500 | Millipore (Ref. 06-570) |
| Secondary antibodies | ||
| Alexa Fluor 488 goat-anti-mouse IgG antibody | 1 : 200 | Molecular Probes, the Netherlands (Ref. A-11001) |
| Alexa Fluor 594 goat-anti-rabbit IgG antibody | 1 : 200 | Molecular Probes, the Netherlands (Ref. A-11012) |
| Anti-mouse IgG biotin conjugate | 1 : 100 | Sigma (Ref. B-7264) |
| Anti-rabbit IgG biotin conjugate | 1 : 50 | Sigma (Ref. B-7389) |