Figure 3.

Detection of viral RNA by real-time reverse transcription–PCR (RT-PCR) from oropharyngeal (A) and cloacal (B) swabs of 5 vaccinated and 5 nonvaccinated falcons after challenge with 10^6.0 50% egg infectious doses of the highly pathogenic avian influenza virus strain A/Cygnus cygnus/Germany/R65/06 (H5N1). Y axis shows cycle-of-threshold (Ct) values of real-time RT-PCRs detecting an M gene fragment in individual swab samples of each animal. Asterisks represent extreme values; open circles show individual outliers; black bars within boxes indicate medians.