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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Free Radic Biol Med. 2009 Aug 14;47(10):1401–1407. doi: 10.1016/j.freeradbiomed.2009.08.006

Figure 6. HPLC chromatograms of the reaction between phenylalanine boronic acid (FBA) and ONOO−.

Figure 6

(A-C): Absorption traces recorded at 220 nm (A), 280 nm (B) and 350 nm (C); (D): Fluorescence traces (excitation at 284 nm, emission at 410 nm). (a) FBA (250 μM); (b) FBA (250 μM) + ONOO− (200 μM); (c) Tyr (100 μM); (d) Tyr (100 μM) + HRP (10 nM) + H2O2 (100 μM); (e) Tyr-NO2 (100 μM). All samples have been incubated for 15 min at room temperature in aqueous solution containing phosphate buffer (0.1 M, pH 7.4) and DTPA (10 μM). To fit the scale, traces e have been scaled down by a factor of 0.1 (panel B) and 0.03 (panel C). (E) and (F), the dependences of the phenylalanine boronate and nitrotyrosine concentrations in reaction mixtures on the amount of ONOO− added to the solution of FBA (100 μM).