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Active-site region showing the H35-P36 interaction in C112D/M121E azurin at pH 7.0 (2.1 Å, a, PDBID: 3NP3) and pH 9.0 (2.25 Å, b, PDBID: 3NP4). 2Fo-Fc maps are displayed at the 1σ level. Nitrogen atoms are blue; oxygen atoms are red.