Skip to main content
. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Nature. 2012 May 30;486(7401):74–79. doi: 10.1038/nature11094

Fig. 2. Loss of neocortical Fezf2 expression and CS axons in E4 knockout mice.

Fig. 2

a, Generation of E4 knockout mice (E4−/−). b, Duplex PCR genotyping of wildtype and mutant alleles using primers P1, P2, and P3. c, Analysis of neocortical Fezf2 expression by quantitative (q) RT-PCR. Normalized to Gapdh, Fezf2 mRNA levels were significantly reduced in the E4−/− compared to heterozygous littermate controls (E4+/−). (P = 2.1×10−6, one-tailed Student’s t-test, n = 3 per genotype). Error bars represent s.e.m. d, A schematic depiction of CS system. e-g, Sagittal (top row) and coronal (bottom row) sections of the pons from wildtype (e), cortex-specific Fezf2 knockout (f), and E4−/− (g) mice immunostained for axon marker L1CAM (red) and PRKCG (green). The near complete loss of CS axons (arrowheads and dashed outlines) in the E4−/− is a phenocopy of cortex-specific Fezf2 deletion. Scale bars represent 200 µm.