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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Nature. 2012 May 30;486(7401):74–79. doi: 10.1038/nature11094

Fig. 4. Sox4 and Sox11 are required for Fezf2 expression and CS tract formation.

Fig. 4

a, The requirement of SOX4 and SOX11 for E4 transactivation was determined in neurons cultured from E14.5 heterozygous littermate control and Sox4;Sox11 cdKO mice and transfected with the E4 luciferase construct. The activity of E4 in double knockout neurons was not significantly above background (1.1 fold. b, Analysis of neocortical Fezf2 expression by qRT-PCR in cortex-specific Sox4 and/or Sox11 mutants. Normalized to Gapdh, Fezf2 mRNA levels were dramatically reduced in cdKO but not Sox4 or Sox11 mutants. c-d, Sagittal (top row) and coronal (bottom row) sections of the control and cdKO pons immunostained for L1CAM (red) and PRKCG (green). The dramatic loss of L1CAM and PRKCG-positive CS tract axons in the cdKO (arrowhead and dashed outline) is similar to that of the cortex-specific Fezf2 mutant. Errors bars represent s.e.m. One-tailed Student’s t-test; NS=Not significant, **P<0.01, ***P<0.001. n = 4 per genotype. Scale bars represent 200 µm.