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. 2012 Apr 3;159(2):558–564. doi: 10.1104/pp.112.195214

Figure 1.

Figure 1.

Analysis of GFP expression in plants infected with PVX-derived transcripts. A, Physical map of the PVX-derived construct used in this work. PVX-based vector P2C2S and cDNAs (GFP, GFP/IV2, and GFP/IV2/ELVd) cloned under the control of the duplicated PVX-CP promoter are shown at top. The bottom shows representations of PVX-GFP, PVX-GFP/IV2, and PVX-GFP/IV2/ELVd(+) chimeric constructs. The constructs are not drawn to scale. B, GFP fluorescence stereomicroscopy images of N. benthamiana leaves inoculated with PVX (unmodified), PVX-GFP, PVX-GFP/IV2, and PVX-GFP/IV2/ELVd(+) transcripts at 7 and 10 d post inoculation. C, Serological detection of GFP. Total proteins were extracted from leaves, electrophoresed by 10% SDS-PAGE (top), and blotted for serological detection (bottom). GFP was clearly detected in both PVX-GFP and PVX-GFP/IV2/ELVd(+) systemically infected plants, confirming the correct processing of the chimeric GFP mRNA carrying the IV2/ELVd(+) insertion. D, RT-PCR amplification of processed and unprocessed GFP mRNAs. E, Confirmation (by RT-PCR) of systemic PVX infection in N. benthamiana plants inoculated with the construct used in this work.