Figure 3.

Analysis of GFP expression in plants infected with PVX-derived transcripts carrying partial-length ELVd sequences. A, GFP fluorescence stereomicroscopy images of N. benthamiana leaves inoculated with PVX-GFP, PVX-GFP/IV2/L-ELVd, PVX-GFP/IV2/R-ELVd, and PVX-GFP/IV2/Up-ELVd transcripts at 6, 9, and 12 d post inoculation (dpi). B, Confirmation (by RT-PCR) of systemic PVX infection in N. benthamiana plants inoculated with the different constructs used in this assay. C, RT-PCR amplification of processed and unprocessed GFP mRNAs.