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SDS-PAGE of TCE purified from tulip petals. Proteins were separated by 12.5% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Lane M, Molecular size markers; lane 1, crude extract; lane 2, DEAE-Toyopearl fractions; lane 3, Butyl-Toyopearl fractions; lane 4, Gigapite fractions; lane 5, Mono-Q fractions; lane 6, Superose 12 fractions.
