Table II. Purification of TCE from tulip petals.
Enzyme activity was measured using 4 mm 6-tuliposide A as substrate.
| Step | Total Activitya | Total Protein | Specific Activity | Yield | Purification |
|---|---|---|---|---|---|
| units | mg | units mg−1 | % | -fold | |
| Crude extract | 3,720 | 418 | 8.90 | 100 | 1 |
| DEAE-Toyopearl | 4,110 | 88.3 | 46.6 | 110 | 5 |
| Butyl-Toyopearl | 2,870 | 16.3 | 176 | 77.2 | 20 |
| Gigapite | 1,420 | 2.85 | 498 | 38.2 | 56 |
| Mono-Q HR 5/5 | 1,360 | 0.624 | 2,180 | 36.6 | 245 |
| Superose 12 | 634 | 0.280 | 2,260 | 17.0 | 254 |
a
One unit of enzyme activity is defined as the amount of enzyme that catalyzes 6-tuliposide A conversion to tulipalin A at a rate of 1 μmol min−1.